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tRNA-uridine 2-thiolation (thermophilic bacteria)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
TtuB_sulfur-carrier_protein + ATP + H+ <=> carboxyadenylated_[TtuB_sulfur-carrier_protein] + diphosphate 2.7.7.M15

L-cysteine + [L-cysteine_desulfurase]-L-cysteine <=> L-alanine + [L-cysteine_desulfurase]-S-sulfanyl-L-cysteine not assigned

[L-cysteine_desulfurase]-S-sulfanyl-L-cysteine + [TtuD_protein]-L-cysteine <=> [L-cysteine_desulfurase]-L-cysteine + [TtuD_protein]-S-sulfanyl-L-cysteine 2.8.1.M4

carboxyadenylated_[TtuB_sulfur-carrier_protein] + [TtuD_protein]-S-sulfanyl-L-cysteine + reduced_acceptor <=> thiocarboxylated_[TtuB_sulfur-carrier_protein] + [TtuD_protein]-L-cysteine + AMP + acceptor + H+ 2.8.1.M3

ATP + thiocarboxylated_[TtuB_sulfur-carrier_protein] + 5-methyluracil54_in_tRNA + H2O <=> AMP + diphosphate + TtuB_sulfur-carrier_protein + 5-methyl-2-thiouracil54_in_tRNA + H+ 2.8.1.15 tRNA-5-methyluridine54 2-sulfurtransferase

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